Curriculum vitae: Julie Støve Bødker

Personal Information

Born Marts 7^th 1978 in Aalborg.
Married to cand. scient., phd. Claus Gyrup Nielsen.
Children: Marie, born 01-10-04 and Dicte, born 11-08-08.
Address:
Søndergårds Allé 35 st tv,
2760 Måløv
Phone no. 28122085 or 86161110
E-mail: julie@boedkers.net

Personal Qualifications

Well organized, focused, industrious and quality conscious.
Patient, flexible, and quickly adapts new knowledge.
A good colleague with positive outlooks and a good sense of humor.

Work Experience

2008 July- 2009: Maternity leave - second child, Dicte.
2007 August- 2010: Postdoc, Clinical Institute, University of Aarhus in the group of Professor Torben F. Ørntoft.

Education

2007 October: Defense of PhD-thesis at the Institute of Molecular Biology, University of Aarhus.
2004 Sep.-2005: May Maternity leave - first child, Marie.
2004 June: Cand. scient in Molecular Biology from University of Aarhus by passed qualification exam.
2003 Sep.-Dec.: Laboratory stay in the group of Professor F.C. Church, School of Medicine, University of North Carolina, USA.
2002 Sep. -2007: June PhD student in the group of Peter Andreasen, Laboratory for Cellular Protein Research, Institute of Molecular Biology, University of Aarhus, with a full financed faculty stipend after the ”4+4”-model. Grade average 10.5 (The 13-scale!).
2001 September: Bachelor degree.
1998 September: Accepted as a student on the Chemistry/Molecular Biology study, University of Aarhus.
1997 June: Mathematical student from Støvring Gymnasium.

Laboratory Experience

Characterization of proteins:

Enzyme assays using chromogenic substrate (activity measurements of proteinase in reactions with inhibitor under various conditions); ELISA; fluorescence-labeling of proteins and studies of the interaction between serine proteinase and inhibitor in a stopped-flow fluorimeter; analysis of protein-protein interactions using surface plasmon resonance in a Biacore; radioactive-labeling of proteins ([32P]).

Expression and purification of proteins:

Culturing of E.coli-, mammalian- and hybridoma-cells; Protein purification using affinity chromatography and size-exclusion chromatography; site-directed mutagenesis with PCR; DNA cloning; generation of cell lines with a stable expression of a chosen protein (using HEK293 Flp-In cells or selection for neomycin-resistant cells); cloning of genes from human RNA using TOPO cloning; transient transfection of COS-7- and HEK293 cells; Western blots of cell extracts.

Cell-assays:

Adhesion-, migration- and invasion-assays; Fluorescence labeling of mammalian cells and quantification using fluorescent microscopy.

Identification of prognostic markers:

Immunohistochemical staining of human bladder cancer tissue sections; statistical analysis of correlation between protein expression in the tumor and the patient response to chemotherapy.

IT-knowledge

Experienced user of Excel and Sigmaplot, the program SwissPDB viewer to display PDB-files of proteins (3D-structures), Word, Internet Explorer, the database PubMed, the drawing program CorelDRAW, and Adobe Illustrator to generate illustrative figures and Powerpoint for presentation of data.

Langue

Native langue: Danish.

Fluency in spoken and written English.

Teaching Experience

I have taught students of molecular biology in the laboratory courses: Biochemistry, Molecular Biology, and Cell Biology from the Institute of Molecular Biology, University of Aarhus.

Extra Courses at the University

PhD-course in Patent, University of Aarhus and Østjysk Innovation, fall 2006.
Biology of Blood Diseases, University of North Carolina, USA (college course), fall 2003.
Heidelberg, EMBL Workshop: Mechanisms of Cell Migration, spring 2003.
PhD-course in Scientific English, University of Aarhus, spring 2003.
Fluorescence Techniques for Biomolecules and Cells, course, University of Southern Denmark, January 2003.
Directed Evolution, PhD-course, University of Aalborg, fall 2002.
Protein structure, PhD- course, University of Aarhus, summer 2002.

Grants

2003: 15.000 kr from ”Harboe Fonden” and 10.000 kr from ”Familien Hede Nielsens Fond”, to laboratory stay in the USA.
2007: 20.000 kr from ”FRITS, GEORG OG MARIE CECILIE GLUDS LEGAT” to materials.
2008: 10.000 kr from ”Annuum”, Clinical Institute, University of Aarhus Klinisk Institut, Aarhus Universitet to materials.
4.500 kr from “Oticon Fonden” to conference in the USA.

Publication List: Julie Støve Bødker

Articles

Mapping of the epitope of a monoclonal antibody protecting plasminogen activator inhibitor-1 against inactivating agents. Bødker JS, Wind T, Jensen JK, Hansen M, Pedersen KE, Andreasen PA (2003). Eur. J. Biochem. 270, 1672-9.
Biochemical mechanism of action of a diketopiperazine inactivator of plasminogen activator inhibitor-1. Einholm AP, Pedersen KE, Wind T, Kulig P, Overgaard MT, Jensen JK, Bødker JS, Christensen A, Charlton P, Andreasen PA (2003). Biochem. J. 373, 723-32
Construction of a plasminogen activator inhibitor-1 variant without measurable affinity to vitronectin but otherwise normal. Jensen JK, Durand MK, Skeldal S, Dupont DM, Bødker JS, Wind T, Andreasen PA (2004). FEBS Lett. 556, 175-9.
Plasminogen activator inhibitor-I and tumour growth, invasion, and metastasis. Durand MK, Bødker JS, Christensen A, Dupont DM, Hansen M, Jensen JK, Kjelgaard S, Mathiasen L, Pedersen KE, Skeldal S, Wind T, Andreasen PA (2004). Thromb. Haemost. 91, 438-49.
Additivity in effects of vitronectin and monoclonal antibodies against alpha-helix F of plasminogen activator inhibitor-1 on its reactions with target proteinases. Komissarov AA, Andreasen PA, Bødker JS, Declerck PJ, Anagli JY, Shore JD (2005). J. Biol. Chem. 280, 1482-9.
Biochemical properties of plasminogen activator inhibitor-1. Dupont DM, Madsen JB, Kristensen TK, Bødker JS, Wind T, Blouse GE, Andreasen PA (2008). Invited review for Frontiers Biosci, in press.

Attended Conferences

Clotting Factor Inhibitors, 20th Anniversary Symposium. Chapel Hill, North Carolina, USA, 18-20 sept. 2003.
Danish Society for Cancer Research, Annual meeting: Poster presentation, 5. may 2006. Titel: Analysis of molecular interactions of plasminogen activator inhibitor-1 by monoclonal antibodies. Authors: Bødker, J.S., Wind, T., Petersen, H.H., and Andreasen, P.A.
Annual meeting in the Institute of Molecular Biology. Poster presentation, 18. August 2006. Titel: Analysis of molecular interactions of plasminogen activator inhibitor-1 by monoclonal antibodies. Authors: Bødker, J.S., Wind, T., Petersen, H.H., and Andreasen, P.A.
Annual meeting in the Institute of Molecular Biology. Poster presentation, 13. April 2007. Titel: Dissecting serpin-protease reaction pathways by the use of monoclonal antibodies, BIACORE, stopped flow fast kinetics and site directed mutagenesis. Authors: Bødker, J.S., Blouse, G.E., Dupont, D.M., Andreasen, P.A.
XI^th International Workshop on Molecular & Cellular Biology of Plasminogen Activation, Stockholm. Poster presentation, 16-20. June 2007. Titel: Dissecting serpin-protease reaction pathways by the use of monoclonal antibodies. Authors: Bødker, J.S., Blouse, G.E., Dupont, D.M., Andreasen, P.A.
American Association for Cancer Research, Annual meeting 2008. San Diego, USA. Poster presentation, 12-16. April 2008. Titel: Identification of new prognostic markers for chemotherapy response in patients with advanced bladder cancer. Authors: Bødker, J.S., Dyrskjøt, L., Hartmann, A., Wild, P., Als, A.B., Ørntoft, T.F. and Birkenkamp-Demtroeder, K.

References

Current employer:: Professor of Clinical Biochemistry, Head of Department, MD Torben F. Ørntoft Institute of Clinical Biochemistry, University of Aarhus. Phone no: 89 49 51 00 E-mail: orntoft@ki.au.dk
Cand. scient and PhD supervisor:: Associate Professor, Dr. scient Peter A. Andreasen Institute of Molecular Biology, University of Aarhus. Phone no: 89 42 50 80, cell phone: 28 99 25 89 E-mail: pa@mb.au.dk